Journal: Developmental cell
Article Title: In vitro and in vivo development of the human airway at single cell resolution
doi: 10.1016/j.devcel.2020.01.033
Figure Lengend Snippet: A) Schematic of the experimental setup and samples included in the analysis. B) A total of 8,443 EPCAM+ cells were computationally isolated and cellular transcriptome heterogeneity was visualized using a tSNE plot revealing 12 clusters of cells. C) Left panel: boxplots (interquartile range with minimum and maximum, outliers removed from plot) show expression level distributions of canonical cell type markers in each cluster. Right panel: heatmap shows z-transformed cluster average expression levels of top 50 cluster markers ranked by log-transformed fold change in expression levels in each cluster compared to other clusters in any of the clusters. Clusters were identified by expression of markers canonically associated with cell populations based on published literature, or given a new name (e.g. secretory progenitor, bud tip adjacent). A list of the upregulated genes in the bud tip progenitor cluster (BP) and the basal cell cluster (BC) with top fold change in expression levels relative to all other clusters is shown and provides a gene signature for these cell populations. Cell cluster names and colors correspond to those in Figure 1B. D) Feature plots of canonical bud tip progenitor markers SFTPC and AGER, a canonical marker of alveolar epithelial type 1 cells. E) Protein staining for SOX9 (green), SFTPC (pink), and DAPI (blue) in bud tips of a 16 week fetal lung specimen. Protein staining for SOX9 (green), PDPN (pink), RAGE (also known as AGER; white), and DAPI (blue) in cells adjacent to the bud tips in a 16 week fetal lung sample. Scale bars represent 50 μm. F) Feature plots showing expression of canonical basal cell markers TP63 and KRT5, as well as KRT15 and IL33, which are highly specific to cluster 7. G) Protein staining of tracheal TP63+ basal cells in 17 week fetal lungs for other basal cell markers identified in cluster 7 by scRNA-seq: KRT15 (pink), EGFR (pink), IL33 (pink), F3 (white) and PDPN (pink).
Article Snippet: Rabbit anti-IL33 , Sigma-Aldrich Atlas Antibodies , HPA024426.
Techniques: Isolation, Expressing, Transformation Assay, Marker, Staining
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